Bhagwati Scientific

HPLC Vials and Septa: An OverviewHPLC vials and septa are essential components for sample containment in High-Performance Liquid Chromatography (HPLC) and GC/MS analysis. They ensure sample integrity by providing an airtight seal, reducing evaporation, and preventing contamination. The vials are typically 2 mL borosilicate glass or polypropylene, while the septa (cap liners) provide a resealable barrier for autosampler needle penetration. Agilent +4HPLC Vials: Types and DescriptionsHPLC vials are chosen based on sample volume, autosampler compatibility, and chemical properties. 

• By Closure Style:
  • Screw Top Vials (9mm/12x32mm): Most popular and user-friendly. They offer an excellent seal and are re-usable, making them ideal for long-term storage.
  • Crimp Top Vials: Provide the tightest, most consistent seal for volatile samples and high-throughput systems, but require crimping/decapping tools.
  • Snap Top Vials: Fast and easy to use without tools, but offer moderate sealing efficiency and are best for short-term analysis.
• By Material:
  • Clear Glass: Standard 3.3 borosilicate glass, compliant with USP Type 1, offering low-expansion and high chemical inertness.
  • Amber Glass: Required for UV-sensitive, photolabile compounds to protect sample integrity.
  • Polypropylene (PP): Ideal for samples that react with glass, or for high-pH/inorganic samples where metal ion leaching is a concern.
• Specialized Vials:
  • Microvials/Inserts: Used for limited sample volumes to maximize retrieval.
  • Headspace Vials: Specifically designed to withstand pressure in GC analysis